Protocols

DNA extractions related protocols:

Qiagen DNeasy blood and tissue sample tweaked protocol for Timema here.

High molecular weight DNA extraction protocol (used for Chromium 10X de novo genome sequencing), here.

Tweaked phenol chloroform DNA extraction (used for Discovar de novo genome sequencing), here.

Regular phenol chloroform DNA extraction (used for whole genome resequencing), here.

How to clean the metal beats, here.

Rad sequencing library preparation:

Library preparation for single end reads in Timema (applied succesfuly in Mytilus galloprovincialis), here.

Library preparation for paired-end reads in Timema (appendix of above protocol), here.

Oligos and barcodes

The sequences of 96 barcoded primers used for whole genome resequencing library preparation in the NERC velocity project, here. The i5 and i7 barcodes are unique to circumvent barcode hoping problems.